Genomic and phylogenetic analysis of a multidrug-resistant Burkholderia contaminans strain isolated from a patient with ocular infection

The genus Burkholderia comprises rod-shaped, non-spore-forming, obligately aerobic Gram-negative bacteria that is found across diverse ecological niches. contaminans, an emerging pathogen associated with cystic fibrosis, frequently isolated from contaminated medical devices in hospital settings. aim of this study was to understand the genomic characteristics, antimicrobial resistance profile and virulence determinants B. contaminans strain SBC01 eye a patient hit by cow's tail. A hybrid sequence isolate generated using Illumina HiSeq Oxford Nanopore Technology platforms. Unicycler used assemble sequence. draft genome annotated NCBI Prokaryotic Genome Annotation Pipeline. Antimicrobial susceptibility testing performed VITEK®2. genes were identified validated bioinformatics tools. assembled size 8 841 722 bp G+C content 66.33% distributed 19 contigs. Strain possess several efflux pump genes. susceptible tetracyclines, meropenem ceftazidime. Many encoding potential factors identified. belonging type 482 (ST482) multidrug-resistant containing genes, revealing risks infections new spp. large G+C-rich has myriad factors, highlighting its pathogenic potential. Thus, while providing insights into uncommon species, present analysis will aid understanding evolution speciation genus.